Pathology Portal

SO5 E217- Episode 217: Micro Minutes: Don't Let PEA Fool You

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PEA agar is often treated as "the gram-positive plate," but that shortcut can lead to real bench-level mistakes. In this MicroMinutes episode, we break down what phenylethyl alcohol agar actually does, why growth on PEA does not automatically mean an organism is gram-positive, and how selective media can change colony appearance and behavior.

You'll hear about:

  • What PEA inhibits — and what it doesn't •
  • Why Pseudomonas aeruginosa can grow quite well on PEA
  • How colony morphology and hemolysis may differ from blood agar
  • Why Lancefield grouping should never be performed directly from PEA PEA is a powerful recovery tool — but only when you understand its limitations. Short, focused, and straight from the bench, this is your Micro minutes.

Stay connected with Let's Talk Micro:

Website: letstalkmicro.com (https://www.letstalkmicro.com/)

Questions or feedback? Email me at letstalkmicro@outlook.com

Resource details

Contributed by: Pathology Portal
Authored by: Luis Plaza, Let's Talk Micro
Licence: © All rights reserved More information on licences
Last updated: 24 December 2025
First contributed: 24 December 2025
Audience access level: General user

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